In 1905, E. G. Conklin published a remarkable fate map of the ascidian embryo. He showed that “all the principle organs of the larva in their definitive positions and proportions are here marked out in the 2-cell stage by distinct kinds of protoplasm.” This study of cell lineage has been the basis for all subsequent research on the autonomous specification of tunicates. The color plates of this study are considered to be some of the best examples of embryological illustration and descriptive anatomy.

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